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Diane Jackel Greene
Facilities manager/Administrator
Location: SI 201
Phone: (216)875-9824
Fax: (216)687-6972
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Manages shared instrumentation within the Center for Gene Regulation in Health and Disease (GRHD).?Responsible for general upkeep, routine maintenance, and quality control of high-end instrumentations.
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If you are planning to use?advanced fluorescence-based Confocal and DeltaVision microscopes, a BD FACSCanto? benchtop flow cytometer and/or any other shared equipment in the Center, please e-mail and/or call Diane Greene.
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Special features?of our?Nikon A1Rsi Laser Scanning Confocal Microscope?include:?High resolution galvano scanner (4096x4096 pixels);?High speed resonant scanner (30 fps at 512x512 pixels);?Transmitted detector for brightfield imaging and Differential Interference Contrast;?Spectral imaging capability (can image GFP and Alexa 488 in same sample);?DIC optics for transmitted light imaging;?Perfect focus system (eliminates focus drift during live imaging);?Motorized XYZ stage with piezo control;?Photoactivation, photobleaching, and FRET capable;?Environmental chamber.
Special features?of our?Deltavision Deconvolution Microscope?include: Filter sets for imaging of DAPI, FITC, Cy3, and Cy5 or similar fluors;?Cooled CCD for high speed, low noise image acquisition;?DIC optics for transmitted light imaging;?Motorized XYZ stage with piezo control;?Workstation for image acquisition and deconvolution (upgraded 2018);?Environmental chamber.
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? ? Nikon A1Rsi?confocal microscope? ? ?DeltaVision deconvolution microscope
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? ? ?BD FACS Canto II Flow Cytometer?? ? ? ? ?Typhoon 9410 imaging scanner
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? ? ? ? Amersham Typhoon 5 Imager? ? ? ? ? ? ? Newly renovated GRHD lab?
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?Beckman Optima L90K ultracentrifuges? ? ? ? Beckman J2-21 centrifuges
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? ? ?Odyssey Fc Imaging System? ? ? ? ? ? ? ? ? ? Thermo Electron French press
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? ? ? ? New cell culture facility? ? ? ? ? ? ? ? ? Beckman LS 6500 scintillation counter
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? ? ? ? AKTA Purifier FPLC System? ? ? ? ?Heska Hematology and Element Analyzers